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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: Cancer Res. 2011 May 17;71(14):5020–5029. doi: 10.1158/0008-5472.CAN-11-0908

Fig. 2. P-STAT3 is regulated by IL6 cytokine family signaling in PDAC cell lines.

Fig. 2

A, Gene expression micorarray data from P-STAT3 high and low PDAC lines were analyzed to identify transcripts differentially enriched in P-STAT3 high cell lines. 126 transcripts found to be enriched in P-STAT3 high cell lines were cross-referenced with 30 positive regulators of STAT3 tyrosine phosphorylation identified by gene ontology search. Only one gene, IL6ST (encoding gp130) was present in both data sets. B, IL6ST transcript level z-score for 15 PDAC cell lines was correlated with drug sensitivity data to 3μM AZ960 from a large cell line repository drug screen. P value and r2 value are shown. C, PDAC cell lines were treated for 24h with increasing concentrations of gp130-neutralizing antibody. Cell lysates were probed with the indicated antibodies. D, Gene expression microarray data from 36 human PDACs and matched normal pancreas controls (normal) were analyzed for expression of IL6 cytokine family members. Bars represent mean of each group. P values are shown (N.S., not significant).