Table 3. PGE2, PGF2α and PGD2 release induced by various agents in h-CM and CHO-B2 cells.
| Compound | PGE2 production in h-CM cells (EC50, nM) | PGF2α production in h-CM cells (EC50, nM) |
|---|---|---|
| BK |
8.9±0.2 |
15.9±9.2 |
| Hyp3-BK |
10.1±4.2 |
9.2±5.3 |
| Lys-BK |
10.3±5.9 |
35.6±16.1 |
| Met-Lys-BK |
32.2±9.0 |
35.6±16.1 |
| RMP-7 |
27.2±9.8 |
18.4±5.2 |
| Des-Arg9-BK |
>1,000 |
>1,000 |
| HOE-140 |
>10,000 |
>10,000 |
| (R)-WIN-64338 |
>10,000 |
>10,000 |
| (S)-WIN-64338 | >10,000 | >10,000 |
| Compound | PGE2 production in CHO-B2 cells (EC50, pM) | PGF2α production in CHO-B2 cells (EC50, pM) | PGD2 production in CHO-B2 cells (EC50, pM) |
|---|---|---|---|
| BK |
32.8±9.03 |
32.1±12.2 |
11.3; 181 |
| RMP-7 |
29.4±16.2 |
26.5±12.8 |
nd |
| Hyp3-BK |
30.7±18.5 |
59.3±37.3 |
30.4; 58.2 |
| Lys-BK |
67.5±27.5 |
71.2±25.1 |
26.3; 64.6 |
| Met-Lys-BK |
139.0±51.3 |
106.0±70.5 |
78.8; 96.6 |
| Des-Arg9-BK |
>100,000 |
>100,000 |
>100,000 |
| HOE-140 |
>10,000 |
>10,000 |
nd |
| (R)-WIN-64338 |
>10,000 |
>10,000 |
nd |
| (S)-WIN-64338 | >10,000 | >10,000 | nd |
Data are mean ± SEM from 3 to 6 experiments for each cell type; nd represents not determined.