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. 2013 Jun 21;8:2273–2284. doi: 10.2147/IJN.S45817

Figure 4.

Figure 4

Prussian blue staining.

Notes: Prussian blue staining of labeled MLECs (B–E) and colon 26 cells (G–J) after incubation with LYVE-1-PEG-USPIO at 25 μg Fe/mL (B and G) and 100 μg Fe/mL (D and I) and PEG-USPIO at 25 μg Fe/mL (C and H) and 100 μg Fe/mL (E and J) for 2 hours. Unlabeled MLECs (A) and unlabeled colon 26 cells (F) as control groups. Bar = 50 μm.

Abbreviations: LYVE-1-PEG-USPIO, lymphatic vessel endothelial hyaluronan receptor-1 binding polyethylene glycol-coated ultrasmall superparamagnetic iron oxide; MLECs, mouse lymphatic endothelial cells; PEG-USPIO, polyethylene glycol-coated ultrasmall superparamagnetic iron oxide.