Table 1.
Iron content and R2 of MLECs and colon 26 cells after incubation with LYVE-1-PEG-USPIO and PEG-USPIO at various concentrations for 2 hours
| Incubation concentration (μg Fe/mL) | Nanoparticle labeled cells | AAS Iron content (pg Fe/cell) | MR imaging R2(s−1) |
|---|---|---|---|
| 0 | MLEC + LYVE-1-PEG-USPIO | 0.93 ± 0.23 | 2.12 ± 0.21 |
| MLEC + PEG-USPIO | 0.95 ± 0.32 | 2.32 ± 0.19 | |
| Colon 26 + LYVE-1-PEG-USPIO | 0.87 ± 0.39 | 2.24 ± 0.23 | |
| Colon 26 + PEG-USPIO | 0.88 ± 0.50 | 2.15 ± 0.25 | |
| 6.25 | MLEC + LYVE-1-PEG-USPIO | 9.32 ± 1.36 | 2.92 ± 0.23 |
| MLEC + PEG-USPIO | 8.16 ± 1.12 | 2.63 ± 0.21 | |
| Colon 26 + LYVE-1-PEG-USPIO | 7.64 ± 1.21 | 2.67 ± 0.34 | |
| Colon 26 + PEG-USPIO | 7.44 ± 1.15 | 2.43 ± 0.28 | |
| 12.5 | MLEC + LYVE-1-PEG-USPIO | 17.52 ± 2.12 | 3.90 ± 0.57 |
| MLEC + PEG-USPIO | 8.92 ± 1.08 | 2.71 ± 0.46 | |
| Colon 26 + LYVE-1-PEG-USPIO | 10.23 ± 1.42 | 3.03 ± 0.59 | |
| Colon 26 + PEG-USPIO | 8.64 ± 1.28 | 2.54 ± 0.35 | |
| 25 | MLEC + LYVE-1-PEG-USPIO | 46.04 ± 3.12 | 8.52 ± 0.34 |
| MLEC + PEG-USPIO | 18.16 ± 2.04 | 3.71 ± 0.24 | |
| Colon 26 + LYVE-1-PEG-USPIO | 19.84 ± 2.16 | 4.85 ± 0.29 | |
| Colon 26 + PEG-USPIO | 15.56 ± 1.36 | 2.97 ± 0.38 | |
| 50 | MLEC + LYVE-1-PEG-USPIO | 101.64 ± 4.24 | 19.43 ± 0.87 |
| MLEC + PEG-USPIO | 30.36 ± 2.72 | 5.72 ± 0.79 | |
| Colon 26 + LYVE-1-PEG-USPIO | 39.12 ± 2.44 | 9.18 ± 0.75 | |
| Colon 26 + PEG-USPIO | 22.92 ± 1.96 | 4.94 ± 0.86 | |
| 100 | MLEC + LYVE-1-PEG-USPIO | 208.24 ± 7.04 | 35.21 ± 1.98 |
| MLEC + PEG-USPIO | 35.24 ± 3.92 | 7.26 ± 0.89 | |
| Colon 26 + LYVE-1-PEG-USPIO | 58.80 ± 4.28 | 12.36 ± 1.21 | |
| Colon 26 + PEG-USPIO | 31.64 ± 2.92 | 6.32 ± 0.98 | |
| 200 | MLEC + LYVE-1-PEG-USPIO | 452.80 ± 12.48 | 62.50 ± 3.06 |
| MLEC + PEG-USPIO | 53.61 ± 4.32 | 12.11 ± 2.42 | |
| Colon 26 + LYVE-1-PEG-USPIO | 101.92 ± 5.40 | 22.33 ± 3.38 | |
| Colon 26 + PEG-USPIO | 47.96 ± 3.44 | 10.35 ± 1.56 |
Note: Results expressed as mean ± standard deviations (n = 3).
Abbreviations: AAS, atomic absorption spectrometry; LYVE-1-PEG-USPIO, lymphatic vessel endothelial hyaluronan receptor-1 binding polyethylene glycol-coated ultrasmall superparamagnetic iron oxide; MLECs, mouse lymphatic endothelial cells; MR, magnetic resonance; PEG-USPIO, polyethylene glycol-coated ultrasmall superparamagnetic iron oxide.