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. 2013 Jun 21;8:2247–2257. doi: 10.2147/IJN.S45059

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© 2013 Kim et al, publisher and licensee Dove Medical Press Ltd

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

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Flow cytometry analysis of the indirect blood samples including OVCAR-3 cells after incubation with MUC1-MNP-SiO₂(RITC).

Notes: (A) Unspiked blood samples. (B) Positive control (5000 OVCAR-3 cells). (C) Indirect blood samples with 100 OVCAR-3 cells. (D) Indirect blood samples with 1000 OVCAR-3 cells. (E) Indirect blood samples with 10,000 OVCAR-3 cells. Numbers of positive cells are shown in parentheses and indicated by red dots.

Abbreviations: OVCAR-3, human ovarian cancer cell line; MUC1, mucin 1 cell surface-associated antibody; P1, population 1; MNP-SiO₂(RITC), magnetic nanoparticle-SiO₂(rhodamine B isothiocyanate); SSC-A, side scatter area; PE-A, phycoerythrin area.