Flow cytometry analysis of the indirect blood samples including DAPI staining of OVCAR-3 cells after incubation with MUC1-MNP-SiO2(RITC).
Notes: (A) Unspiked blood samples. (B) Indirect blood samples with 100 OVCAR-3 cells. (C) Indirect blood samples with 1000 OVCAR-3 cells. (D) Indirect blood samples with 10,000 OVCAR-3 cells. Numbers of positive cells are shown in parentheses.
Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole fluorescent stain; OVCAR-3, human ovarian cancer cell line; MUC1, mucin 1 cell surface-associated antibody; MNP-SiO2(RITC), magnetic nanoparticle-SiO2(rhodamine B isothiocyanate); DAPI-A, 4’,6-diamidino-2-phenylindole area; PE-A, phycoerythrin area; P1, population 1; P2, population 2; P3, population 3.