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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: Methods Mol Biol. 2012;905:213–224. doi: 10.1007/978-1-61779-949-5_13

Figure 1. Protocol for RNase footprinting analyzed by primer extension.

Figure 1

The accessible single stranded regions of assembled RNPs are exposed to RNase under single-cleavage conditions. cDNAs are generated by primer extension of the RNase treated RNA using 32P-labeled primers. The cDNAs are resolved on a polyacrylamide sequencing gel and regions of protection from RNase cleavage are identified