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. 2013 Jun 20;13:279. doi: 10.1186/1471-2334-13-279

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Copyright © 2013 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cell populations in granuloma-like aggregates. May–Grünwald–Giemsa (MGG) staining showed that there are mainly macrophages, ECs (A, D) and MGCs in the aggregates (B, D). MGCs were formed by the intercellular fusion and phagocytosis of cells (C). M. leprae were stained with Ziehl-Neelsen (shown with arrows) and the bacilli were found to be restricted to the central cytoplasmic region of the MGCs (E, F). Confocal microscopy of MGCs showed M. leprae stained with auramine O (green) and the nuclei stained with Hoescht (G, H).