Table 2.
Primers used to amplify short-PCR fragments from Ascaridia galli, Ascaridia columbae and Ascaridia sp
| Name of primer | Sequence (5′ to 3′) | Reference |
|---|---|---|
|
For A. galli |
|
|
|
p cox 1 |
|
|
| AGCox1F |
GAAGTTTGTATTTGACTGGTAAGAA |
This study |
| AGCox1R |
CAGTGAGACCACCAATAGTAAACAA |
This study |
|
p nad 1 |
|
|
| JB11 |
AGATTCGTAAGGGGCCTAATA |
[36] |
| JB12 |
ACCACTAACTAATTCACTTTC |
[36] |
|
p nad 4 |
|
|
| AGNad4F |
CTTATTATTTAATTTTTTATGCTGCT |
This study |
| AGNad4R |
AAGCGGCTAAAGCCTTAGCATCACT |
This study |
|
For A. columbae and Ascaridia sp. |
|
|
|
p cox 2 |
|
|
| ACCox2F |
TTTAAGTTTGTTGTATTATTATGGTTT |
This study |
| ACCox2R |
AAAATACACCAACCACAGGAAAACT |
This study |
|
p nad 1 |
|
|
| JB11 |
AGATTCGTAAGGGGCCTAATA |
[36] |
| JB12 |
ACCACTAACTAATTCACTTTC |
[36] |
|
p cox 3 |
|
|
| ACCox3F |
TGGTATTTTCTGGACTTTTTTTGAT |
This study |
| ACCox3R | CCAAACTACATCTACAAAATGCCAA | This study |