Skip to main content
. 2013 Jun 21;14:414. doi: 10.1186/1471-2164-14-414

Table 3.

Primers used to amplify long-PCR fragments from Ascaridia galli, Ascaridia columbae and Ascaridia sp

Name of primer Sequence (5′ to 3′)
For A. galli
 
AGC1N1F
ATAGAAGTTTGTATTTGACTGGTAAGAAGGAGGT
AGC1N1R
CACAATACCAGTAACCAAAGTAGCATAAACAG
AGN1N4F
TAAGTTGTTGAAGAAGGAGCAGGAGAGT
AGN1N4R
CAAAAATGGAAAAGAACACAAAGCAGCA
AGN4C1F
TTTTTATGCTGCTTTGTGTTCTTTTCCA
AGN4C1R
CCAAATAAAGTTGCCAGCCACCTAAA
For A. columbae
 
ACC1N1F
AGTTGGACTGTTTATCCGCCTTTGA
ACC1N1R
ATTTCATAAGACACTCTCTGACCTC
ACN1C3F
GCCAGAGGTCAGAGAGTGTCTTATG
ACN1C3R
CTTGCTTCACTATACTCTATTGCCTGT
ACC3C2F
CAGGCAATAGAGTATAGTGAAGCAA
ACC3C2R
ATAGAAGGCACAGCCCAAGAATGAA
ACC2C1F
TAGTATGTGATGTTTGGGAATGCTT
ACC2C1R
CTTTTACACCAGTAGGCACAGCGAT
For Ascaridia sp.
 
ACC1N1F
AGTTGGACTGTTTATCCGCCTTTGA
ACC1N1R
ATTTCATAAGACACTCTCTGACCTC
ASN1C3F
GGTCAGAGGGTTTCTTATGAGATTGCT
ASN1C3R
CAACCGAACAATCTTTATTACTCAACA
ASC3C2F
TGTTGAGTAATAAAGATTGTTCGGTTG
ASC3C2R
ACCAGGAATGTCACCATACTCATAACT
ASC2C1F
TGGAGGTTGATAATCGTTGTGTTTTGC
ASC2C1R CACAACAACAAAGGCTGAAAAACCATC