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. 2013 Jun 26;8(6):e67086. doi: 10.1371/journal.pone.0067086

Figure 4. Modulation of total splenic cellularity for isoflurane and pentobarbital anesthetized rats.

Figure 4

The total splenic cellularity from rats anesthetized with isoflurane or pentobarbital was determined using counting with Trypan blue in Malassez cell for the different groups (control, sham 3 h, VNS 3 h). n = 10 rats per groups. *: p<0.05. A significant decrease was observed for the VNS 3 h group compared to the sham 3 h and control groups (p<0.05) for the isoflurane anesthetized rats. In contrast, no significant effect was observed for the pentobarbital anesthetized rats.