Fig. 4.

IL-23p19 promoter is bound by di- and tri-methylated H3K4 in aged DCs. Cells were stimulated and treated as described in Fig. 2. Chromatin was isolated and immunoprecipitated with H3K4me2 or H3K4me3 antibody. (A) DNA was recovered from the immunoprecipitated and input samples and then analyzed for the presence of IL-23p19 promoter sequences by PCR. Data from four mice each group are shown. (B) Band intensities were analyzed as described in Fig. 2. Data are the mean ± SEM and are representative of three experiments. *Statistically (P ≤ 0.05) significant (young vs. old). (C) Real-time PCR analysis. Data represent the mean ± SEM of three experiments.