Figure 2. Priming of Th1 CD4⁺ T cells with anti-DEC-LmSTI1a in vivo requires targeting to DEC⁺ DCs.

(A) C57BL/6 mice were intraperitoneally immunized with different doses of anti-DEC-LmSTI1a, control Ig-LmSTI1a, or non-targeted LmSTI1a protein (0.1–10 µg) in the presence of 50 µg poly ICLC and 25 µg anti-CD40 (Adj). Fourteen days after immunization, splenocytes were restimulated in vitro with a reactive LmSTI1a peptide mix for 6 h in the presence of BFA. Intracellular staining was performed to detect IFN-γ production in CD3⁺ CD4⁺ T cells. The mean ± SEM (n = 6). (B) As in A, but C57BL/6 wild-type or DEC^−/− mice were immunized with 10 or 1 µg of anti-DEC-LmSTI1a mAb plus Adj. Plots are representative of two experiments.