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. 2013 Jun 26;8(6):e67453. doi: 10.1371/journal.pone.0067453

Figure 5. Anti-DEC-LmSTI1a mAb elicits protection against L. major.

Figure 5

Balb/c mice were vaccinated in a prime-boost regimen consisting of two subcutaneous doses, administered 1 month apart, with 10 µg or 1 µg of control Ig-LmSTI1a or anti-DEC-LmSTI1a and 10 µg of anti-DEC-LACK or anti-DEC-LeIF mAbs in the presence of 50 µg poly ICLC. Ten to 15 days after boost, the mice were intradermally injected in the ear pinnae with 200–1000 L. major metacyclic promastigotes. Quantification of parasites obtained from ear-infected draining LN (A) or per infected ears (B) were calculated 12 weeks after challenge. Shown is the mean ± SEM (n = 5–12, except PBS, n = 2). (C) As in B, but Balb/c mice were intradermally challenged with 200–1000 L. major metacyclic promastigotes 12 weeks after the last immunization. Shown is the mean ± SEM (n = 5, except PBS, n = 3).