Table 5.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description or relevant genotype | Source or reference |
|---|---|---|
| Bacterial strains | ||
| NCTC 11168 |
Campylobacter jejuni wild-type strain |
ATCC* |
| JL272 |
NCTC 11168 derivative erythromycin resistant strain |
Jun Lin [20] |
| KOp50Q |
NCTC 11168 Δcj1169c-cj1170c::cat mutant |
This study |
| KO423Q |
NCTC 11168 Δcj0423-cj0425::aphA3 mutant |
This study |
| KO39Q |
NCTC 11168 Δcj0309c-cj0310c::cat mutant |
This study |
| KO73Q |
NCTC 11168 Δcj1173-cj1174::aphA3 mutant |
This study |
| DKO01Q |
NCTC 11168 Δcj0309c-cj0310::cat, Δcj1173-cj1174::aphA3 mutant |
This study |
| Comp50Q |
KOp50Q complementation strain; Δcj1169c-cj1170c::cat, ITS1::cj1169c-cj1170c complementation strain |
This study |
| ComDK01Q |
DKO01Q complementation strain; Δcj0309c-cj0310::cat, Δcj1173-cj1174::aphA3, ITS1::cj1173-cj1174 |
This study |
| DH5α |
Escherichia coli strain for plasmid propagation |
Invitrogen |
| Plasmids | ||
| pUC19 |
Polymerase chain reaction cloning vector, AmpR** |
Promega |
| pRR |
Complementation cloning vector |
Karlyshev [40] |
| pRRK |
pRR with aphA3; KanR** |
This study |
| pRRT |
pRR with tetO; TetR** |
This study |
| pUp50Q |
cj1169c-cj1170c::cat cloned into pUC19 |
This study |
| pU423Q |
cj0423-cj0425::aphA3 cloned into pUC19 |
This study |
| pU39Q |
cj0309c-cj0310c::cat cloned into pUC19 |
This study |
| pU74Q |
cj1173-cj1174::aphA3 cloned into pUC19 |
This study |
| pRRKp50Q |
cj1169c-cj1170c cloned into pRRK |
This study |
| pRRK39Q |
cj0309c-cj0310c cloned into pRRK |
This study |
| pRRT73Q | cj1173-cj1174 cloned into pRRT | This study |
*ATCC: American type culture collection (ATCC number for this strain is 700819).
**AmpR: Ampicillin resistance, KanR: Kanamycin resistance, TetR: Tetracycline resistance.