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. 2013 Jun 26;8(6):e67855. doi: 10.1371/journal.pone.0067855

Figure 5. Beta-actin binds to specific regions of the Gata2 gene.

Figure 5

(A) Immunoprecipitation with the anti beta-actin and anti gamma-actin antibodies followed by qRT-PCR with 9 specific primers for the Gata2 promotor region yielded 2 loci of interest: amplicon 3 and 8. Error bars represent mean ±SEM; *P<.05, **P<.01. (B) Genomic alignment with multiple species showing that amplicon 3 is partly overlapping with a highly conserved region in the Gata2 promotor, especially in mammals. Also the localization of the specific amplicons relative to the mouse Gata2 gene is shown. Amplicon8 is located between exons 1 and 2. Figure was made using UCSC genome bioinformatics software (http://genome.ucsc.edu/).