Figure 1. Effect ofAcanthoeba castellanii on the frequency of phlD, hcnAB and prnD positive Pseudomonas (A–C) and on the average number of genes per bacterium (D) during the microcosm experiment with barley (means ± SE).
Presence of each gene was tested by colony–PCR on isolates growing on the Pseudomonas specific Gould's S1 medium. closed symbols: bacterial communities co-cultivated with Acanthamoeba castellanii, open symbols: control treatment without protozoa.