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. 2013 Jun 26;8(6):e67689. doi: 10.1371/journal.pone.0067689

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© 2013 Visvanathan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Fluo4 fluorescence images showing an increase in free intracellular calcium levels on treating the cells with Ca²⁺ ionophore. (B) FLIM images showing a decrease in fluorescence lifetime of Hela^H2B-2FP cells that have been treated with Ca²⁺ ionophore. (C) Chart showing the mean lifetime values of individual Hela^H2B-2FP cells before (blue) and after treating with Ca²⁺ ionophore. (Scale bar –10 µm).