Figure 8. Preventing increase in free calcium after ATP depletion does not prevent chromatin compaction.

Increase in free intracellular calcium upon ATP depletion can be prevented by preloading the cells with BAPTA, an intracellular Ca²⁺ chelater. Fluo4 fluorescence in HeLa cells preloaded with BAPTA does not change with ATP depletion, indicating no changes in the intracellular calcium concentration (p-value = 0.72). Increase in compaction of chromatin after ATP depletion (yellow arrow head) is seen from Hoechst staining. (Scale bar 10 µm).