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. 2013 Jun 26;8(6):e66902. doi: 10.1371/journal.pone.0066902

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© 2013 McArt et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Validation of cotinine as the top candidate to suppress cell proliferation phenotype induced via androgen pathway. A, Cells in 6 well plate format were treated with vehicle or various doses of cotinine for 96 hours. Total viable cell numbers were counted by haemocytometer. Cell counts are represented as relative to the untreated control value. B, Cells were treated with indicated doses of cotinine and seeded in xCELLigence 16 well E-plates. Real-time analysis of cell doubling rates was recorded and rate of doubling between 72–96 hours was plotted relative to untreated control using the system software. The experiment was repeated three times with similar results. ** denotes using unpaired two-tailed t-test.

Inline graphic — Validation of cotinine as the top candidate to suppress cell proliferation phenotype induced via androgen pathway. A, Cells in 6 well plate format were treated with vehicle or various doses of cotinine for 96 hours. Total viable cell numbers were counted by haemocytometer. Cell counts are represented as relative to the untreated control value. B, Cells were treated with indicated doses of cotinine and seeded in xCELLigence 16 well E-plates. Real-time analysis of cell doubling rates was recorded and rate of doubling between 72–96 hours was plotted relative to untreated control using the system software. The experiment was repeated three times with similar results. ** denotes using unpaired two-tailed t-test.