Table 1. Detailed information about primers used for qRT-PCR variation.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Tm (°C)	Product (bp)
CALM	GAATTGAAGGAAGCGTTTAA	TGGCCAGCATCATTCTCACA	58	200
SUS	GCTGATGCTGAATGATCGGA	CATCAGGTATTAAACGGGTC	56	357
GA20ox	TGTCTCCTAATGGATTCAAC	CTCGTCACCGTTGAGGAATC	52	225
ERF	GGAATGGAAGGGTCCGATGT	CATGAGATACACTTTCTAACT	52	354
bZIP	CGCCACCTTGAGTCAGCTGT	CAGTGTCTGGGAGTCGGTCA	55	276
WRKY	CTCTTTATCCAGCATCTTTGA	GGTTCTCTCTGCCGTTTCTG	54	348
SAUR	ATGGCTCTCAGGAAATCAAA	ATAGTGAGACCCATGTCGTG	53	266
CBF	AGAGGAGTCAGGGAGAGGA	GCAACCTTCTCTTCGCTCTC	57	329
GBSS	TGGGGAACACTTCAATATCT	CTCCCGAAGAGGAAGACCA	52	307
CONSTANS	GGCATTCCTTGCGATTTCTG	TCGGAGCTGCAATTGTCG	55	182
GIGANTEA	AGAACTCAAGCCACCTACTA	GGTAGCAGTTTCATAGCGAG	53	153
MADS	CTGAGGTCGCTGTGATCGT	GCCTTAAGTTTGGTGTGTTC	52	178
Dof	CAATCTTTCCCAACCTCG	ACGAAGACAAGGACGATGA	53	168
HD	CGCCGTCTGCTCCAACAA	CGATGAAAGTTCCTGTGGCA	60	200
Lipoxygenase	CCCTTTGCCTCAAGTTATCC	GCAGCCCATTCAGGTTATTC	55	206
GP	AGGTTTGGGCACTACTATGG	TTAGGGTATCATCATGCGTG	54	173
PHYB	GTGGAATGCAGCAATGGA	ATCCACATATTTTCCTTTCC	51	211
APETALA	GTCAGTCGGAACCTCTTGC	CCGCCCAAATAAACCTGC	54	175

All primers used for qPCR were designed according to CDS obtained in this study in lotus root.