Table 2. Categorization and abundance of tags.
L1 | L2 | L3 | L4 | ||
Raw Tag | Total number | 4722270 | 4654357 | 4931007 | 4975127 |
Distinct Tag | 327019 | 312178 | 363183 | 385558 | |
Clean Tag | Total number | 4542104 | 4474755 | 4777919 | 4750348 |
Distinct Tag number | 151282 | 137476 | 215872 | 166005 | |
All Tag Mappingto Gene | Total number | 3593944 | 3550079 | 3833530 | 4043129 |
Total percentage of clean tag | 79.13% | 79.34% | 80.23% | 85.11% | |
Distinct Tag number | 91450 | 81334 | 115876 | 108718 | |
Distinct Tag percentage of clean tag | 60.45% | 59.16% | 53.68% | 65.49% | |
Unambiguous TagMapping to Gene | Total number | 2918451 | 2919531 | 3315975 | 3300501 |
Total percentage of clean tag | 64.25% | 65.24% | 69.40% | 69.48% | |
Distinct Tag number | 73493 | 64898 | 93692 | 87374 | |
Distinct Tag percentage of clean tag | 48.58% | 47.21% | 43.40% | 52.63% | |
All Tag-mappedGenes | Total number | 26261 | 24551 | 29314 | 27666 |
Percentage of reference genes | 53.54% | 50.05% | 59.76% | 56.40% | |
Unambiguous Tag-mapped Genes | Total number | 20471 | 18785 | 23448 | 21778 |
Percentage of reference genes | 41.73% | 38.30% | 47.80% | 44.40% | |
Unknown Tag | Total number | 948160 | 924676 | 944389 | 707219 |
Total percentage of clean tag | 20.87% | 20.66% | 19.77% | 14.89% | |
Distinct Tag number | 59832 | 56142 | 99996 | 57287 | |
Distinct Tag percentage of clean tag | 39.55% | 40.84% | 46.32% | 34.51% |
About 5.0 million tags were sequenced, and clean tags and distinct tags were obtained after removal of low quality tags from each library. Some distinct tags were unambiguous tags after mapping to the reference genes, and some were unambiguous tag-mapped genes.