Skip to main content
. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: Curr Drug Targets. 2012 Aug;13(9):1121–1130. doi: 10.2174/138945012802002410

Figure 9.

Figure 9

Correlation of outer- (OM) and inner-membrane (IM) permeabilizing activity. OM permeabilizing activity was determined using E. coli ML-35p (parent ATCC 43827 transformed with pBR322 vector encoding periplasmic β-lactamase); the leakage of periplasmic β-lactamase activity was quantified using nitrocefin as a chromogenic substrate. IM permeabilizing activity was determined using E. coli ML-35 using o-nitrophenyl-α-D-galactopyranoside as the substrate to determine the α-galactosidase activity. Polymyxin B, Polymyxin B nonapeptide (PMBN) and melittin were used as reference compounds.