Figure 16.1.

CHOP expression and annexin staining of FC-loaded primary macrophages. Peritoneal macrophages were elicited and incubated in macrophage medium. At 90% confluence, cells were either loaded with AcLDL or alternatively FC-loaded (AcLDL + 58-035) and (A) immunoblotted for CHOP at indicated times. Blots were stripped and reprobed for actin as a protein loading control. (B) Monolayers were in parallel stained with annexin V (green) and propidium iodide (red) at 16 h to detect mid-stage and late-stage apoptosis. The graph is a quantitation of annexin V-positive, propidium iodide-negative (apoptotic) macrophages in AcLDL cholesteryl ester (CE)-loaded, or FC-loaded macrophages. ^★p < 0.05.