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Staining TUNEL-positive macrophages from PFA-fixed paraffin-embedded sections. (A) Representative micrographs show TUNEL-positive signal (red) in nuclei (blue) of aortic root lesions from lesions. Blue is Hoechst nuclear dye. Green is F4/80. (B) Enumeration of TUNEL-positive F4/80+ macrophages from early (4 week Western Diet fed) and advanced (12-week Western+Diet fed) lesions. ★p < 0.05.
