Fig. 2.

Fluorescence micrograph of the immunocytochemical localisation of occludin and claudin-5 in P.1 PBEC and porcine brain microvessels. P.1 PBEC were grown on glass cover slips (A, B) then stained for tight-junction proteins occludin (A, scale bar: 50 μm) and claudin-5 (B, scale bar: 20 μm). Porcine brain microvessels were isolated from fresh porcine brain tissue onto glass coverslips using the ‘tissue print’ method (Section 4.5). (C) Occludin (viewed at 40×magnification); (D) claudin-5 (20×magnification); nuclei counterstained with Hoescht 33258.