Supplementary Fig. 4.

Proliferation was altered when microglial phenotype was polarized, LPS inducing and IL-4 reducing Ki67 expression. Microglia were stained with Ki67 (red) and Hoechst (blue) after exposure for 12 or 36 h to LPS or IL-4, with PBS as control. Ki67 positive cell numbers were counted and expressed as a proportion of total cell number (Hoechst positive). Photomicrograph were taken at 60× magnification, scale bar 100 μm.