Figure 7.

Knockdown of Cplx4a in pineal photoreceptors did not affect either outer segments or photoreceptor ribbon synapses. A, Cplx4a immunostaining (green) in the synaptic plexus of 2-dpf zebrafish pineal body after injection of control morpholino (MO) at the one-cell stage. The cone-like photoreceptors of the pineal organ are labeled with antibody zpr 1 (red). B, Cplx4a immunostaining was largely absent in the pineal body of 2-dpf zebrafish injected at the one-cell stage with an antisense morpholino directed against Cplx4a mRNA. C, D, Cone-like outer segments of pineal photoreceptors were unaffected by Cplx4a knockdown. E, F, Photoreceptor synapses marked by ribbons in the synaptic plexus of the pineal organ of 2 dpf embryos injected with control morpholino or Cplx4a morpholino. Synaptic ultrastructure was similar in both morphants.