Figure 8.

Absence of photoreceptor outer segments in the retina after knockdown of Cplx4a in larval zebrafish. A, B, Cone photoreceptors were immunostained with antibody zpr1 (red) in sections of 5-dpf retina from control morphants (A) and Cplx4a morphants (B). Synaptic ribbons were stained with an antibody against the ribbon protein, RIBEYE-b (green). Outer segments of cone photoreceptors were visible with zpr1 staining in control morphants (A, arrow) but not in Cplx4a morphants (B, arrow). C, D, Low-magnification electron micrographs of the outer retina in eyes from control morphants (C) and Cplx4a morphants (D) at 5 dpf. OPL, outer plexiform layer; ONL, outer nuclear layer; OS, cone photoreceptor outer segments; RPE, retinal pigmented epithelium. Outer segments were not present in photoreceptors of Cplx4a morphants.