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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Mol Microbiol. 2013 Jun 5;89(1):113–122. doi: 10.1111/mmi.12262

Fig. 4. YlyA affects the efficiency of spore germination.

Fig. 4

Germination kinetics of purified spores were determined by measuring the formation of the fluorescent complex between terbium (Tb3+) and the DPA released from spores. (A) Germination was induced with different millimolar concentrations of L-valine, which is specific for the GerA receptor. Results are plotted as the velocity of DPA release (relative fluorescence units per minute (RFU/min)). (B) Germination was induced with different millimolar concentrations of L-asparagine in the presence of 10 mM each of glucose, fructose and potassium chloride (AGFK), which together are specific for the GerB and GerK receptors. Results are plotted as the velocity of DPA release (RFU/min). (C) Germination was induced with 0.8 mM dodecylamine, which acts on the SpoVA protein channel, and DPA release was measured over time. Results are plotted as the total release of DPA (RFU). The symbols used are: (●), wild-type; (△), ylyA mutant; and (□) ylyA overexpression.