All of the genes encoding the 41 targeted ribosomal proteins in the E. coli genome were disrupted in the presence of a complementing plasmid. Of these, 39 genes were disrupted in the presence of pCDSSara-RP (RP corresponds to the protein name encoded by the gene) which expresses the ribosomal protein in the presence of arabinose induction. rplL and rpsR (encoding L12 and S18, respectively) were disrupted in the presence of pCDSStrc-L12 or S18, respectively, which expresses the protein in the presence of IPTG induction. Spc, specitinomycin; Sm, streptomycin.