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. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: J Mol Biol. 2011 Sep 12;413(4):751–761. doi: 10.1016/j.jmb.2011.09.004

Fig. 2. Growth analysis of deletion strains.

Fig. 2

(a) Growth dependence of the deletion mutants on the complementing genes. All strains contained a genomic deletion (Δ) and a plasmid (p) copy of the ribosomal protein gene indicated. Overnight cultures of the mutants grown in the presence of 0.2% arabinose (for all except ΔrplL pL12 and ΔrpsR pS18) or 1 mM IPTG (for ΔrplL pL12 and ΔrpsR pS18) were diluted 100-fold, spotted on LB agar supplemented with antibiotics and either the inducer (+) or 0.2% glucose (−), and incubated for 15-36 h at 37 °C. (b) Temperature dependence of knockout strains lacking the complementing plasmid. pCDSSara plasmids containing nonessential ribosomal protein genes were removed by sacB-based counterselection, and overnight cultures of the resulting cells were diluted 100-fold, spotted, and grown on LB agar for 48 h at various temperatures.