TABLE 1.

Microarray analysis of gene expression in ASFV-infected macrophage cell cultures

Gene name or function	cDNA accession no.	Best-hit accession no.	Pr4Δ35-Pr4 fold increase at 3 hpi	t-test P value	Avg real-time PCR fold increase at 3 hpi (SD)	Pr4Δ35 supernatant-Pr4 fold increase at 3 hpia	Pr4Δ35-Pr4 fold increase at 6 hpi	Pr4-mock infection fold increase at 3 hpi
Upregulated >2-fold
Cytokine IP-10	CB469489	AB099892	6.89	0.001627	11.55 (9.47-14.08)	1.25	2.21	1.60
Unknown	CB469321		5.45	3.75 × 10−4		1.16	4.01	1.04
Tetratricopeptide repeats, IFIT1	CB469191	P09914	4.84	2.95 × 10−4		1.58	1.89	1.06
Ubiquitin-like 17-kDa protein	CB471836	P05161	4.46	0.001364		1.72	5.76	1.41
Unknown	CB469595		4.06	7.56 × 10−4	5.88 (5.32-6.49)	1.23	2.45	1.20
Unknown	CB468971	U34605	3.03	0.007793		1.24	2.19	0.80
Unknown	CB481699		2.77	0.002065		1.08	2.45	1.15
Ubiquitin ligase like	CB473195	AC020571	2.74	0.001847		1.45	1.94	1.17
ISG43, ubiquitin-specific protease 18	CB470082	AF134195	2.62	0.002544		1.13	1.45	1.08
Unknown	CB479057		2.54	0.008026		1.43	2.04	1.10
LINE1 reverse trancriptase	CB476326	S80119	2.51	0.040625		1.21	1.59	1.24
Unknown	CB478726		2.49	0.025735		1.13	2.45	0.83
PACT, p53-associated protein	CB476189	AF352051	2.38	0.026206		1.31	1.73	1.06
Unknown	CB479458	BF078260	2.34	0.011609		1.14	1.58	1.00
Unknown	CB482476	BI338737	2.32	0.008283		0.99	1.70	1.15
Unknown	CB480284	BG732648	2.30	0.019733		1.25	1.47	1.19
Unknown	CB470560	BI404223	2.28	0.002683		1.37	1.95	1.11
Unknown	CB482594	BF442191	2.27	0.003725		1.26	2.39	1.15
Unknown	CB480019	BE030527	2.23	0.004962		1.63	1.60	1.10
MCP-1	CB482611	Z48479	2.23	0.039477	2.81 (2.18-3.62)	1.57	2.21	1.53
MXB, GTP binding	CB472512	P20592	2.19	0.001413	2.85 (2.37-3.42)	0.82	1.44	1.66
Unknown	CB470650		2.17	5.39 × 10−4		1.24	1.95	1.41
Unknown	CB480279	AC104718	2.16	0.007469		1.56	1.61	1.2
N-Acetylglucosamine kinase	CB482694	AJ242910	2.15	0.069		0.94	2.16	1.00
Unknown	CB473300		2.15	0.006824		1.47	1.45	1.12
Unknown	CB480200	BF442147	2.14	0.027229		1.91	1.63	1.16
Unknown	CB480314	AW619120	2.12	0.004569		1.23	1.67	1.32
RNA helicase DEAD box protein	CB469189	BC014949	2.11	0.006565		1.40	1.75	1.07
Unknown	CB480289	AW436409	2.08	0.010568		1.04	1.85	1.21
Tyrosine phosphatase motif	CB476178	NM_080922	2.04	0.044404		1.42	1.32	1.19
IFN-induced TM-3	CB476806	AF227948	2.03	1.68 × 10−4		1.30	2.01	1.11
Sprouty 2	CB474265	NP_005833	2.03	1.42 × 10−4		1.31	1.59	1.09
Unknown	CB480567		2.03	0.01083		0.99	1.78	1.53
Unknown	CB481133	BI097843	2.02	0.038255		1.29	2.45	1.05
Unknown	CB475887		2.01	0.007619		1.31	1.24	0.91
Unknown	CB481843	BM724068	2.01	0.024038		1.00	1.90	1.12
Unknown	CB479033	AW415288	2.01	0.04682195		1.40	1.97	1.13
Unknown	CB471021	BF078276	2.00	0.00444		1.61	1.32	1.10
No change or down-regulated
ISGF-3 91-kDa component, STAT1	CB474498	P42224	1.74	0.003545		1.12	1.73	0.99
IFN-α/β receptor B chain	CB472490	Q95141	0.92	0.072824	1.40 (1.35-1.46)	0.52	0.83	1.16
p58 inhibitor of PKR	CB472968	U28424	0.46	7.24 × 10−6		0.43	0.35	3.87
IL-8		AB057440	No data	No data	0.703 (0.39-1.07)	No data	No data	No data
IL-1α	CB474300	P18430	0.83	0.03737		No data	1.07	0.99
IL-1β	CB473056	M86725	1.29	0.0488191		No data	0.87	2.63

^aVirus-depleted supernatant was prepared by membrane filtration (MOI of 0.00004).