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. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: Microvasc Res. 2011 Oct 19;83(2):185–193. doi: 10.1016/j.mvr.2011.10.003

Fig. 6.

Fig. 6

Maxadilan induces human neutrophil migration. A. Maxadilan induces human neutrophil migration in a dose dependent fashion: freshly isolated cells (5×105) were assayed for chemotaxis in response to maxadilan at the indicated concentrations. Medium alone (random migration) was used as control (Ctrl) and fMLP (100 nM) were used for comparison. Data represent mean±SD of the number of neutrophils per 1000× field counted under optical microscopy. B. M65 inhibited neutrophil migration induced by maxadilan but not by IL-8: Neutrophils were treated for 60 min with M65 (160 nM), after washing, cells were assayed for chemotaxis in response to IL-8 (30 nM) Data represent mean±S.E.M of the number of neutrophils per 1,000x field counted under optical microscopy. Data derived from three independent experiments. (*) p<0.001 compared with Ctrl; (#) p<0.05 compared with maxadilan alone. C. Reparixin inhibited neutrophil migration induced by maxadilan and IL-8: Neutrophils were treated for 15 min with reparixin 1 μM (RPTX), after washing; cells were assayed for chemotaxis in response to maxadilan (100 ng/mL). Data represent mean±S.E.M of the number of neutrophils per 1,000x field counted under optical microscopy. Data derived from three independent experiments. (*) p<0.001 compared with Ctrl; (#) p<0.05 compared with maxadilan alone.