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. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: Cancer Prev Res (Phila). 2012 Jul 10;5(10):1183–1194. doi: 10.1158/1940-6207.CAPR-12-0201

Figure 5.

Figure 5

Pioglitazone induces 15-PGDH and BRCA1, and suppresses aromatase levels in mouse mammary gland. Mice were fed control diet or control diet supplemented with 0.05% or 0.1% (w/w) pioglitazone for 2 weeks before being sacrificed. Levels of aromatase activity (A), aromatase mRNA (B), 15-PGDH mRNA (C), PGE2 (D) 13,14-dihydro-15-keto PGE2 (E), and BRCA1 mRNA (F) were measured in mammary glands. A, aromatase activity is expressed as femtomoles/μg protein/h. Total RNA was prepared and aromatase (B), 15-PGDH (C), and BRCA1 (F) mRNA levels were quantified by real-time PCR. D and E, PGE2 and 13,14-dihydro-15-keto-PGE2 levels were measured in mammary glands. The biomarker levels under each experimental condition (n = 10) were summarized in terms of mean ± SD ■, P < 0.1; *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus mice that received control diet.