TABLE 2.
MuLV Gr-1.4 integration sites in receptors and signaling-related molecules
| Genea | Virus integration site localization | Databaseb | Tumorsc | Subtype of (leukemia)d | Chromosome
|
Gr-1.4 virus unique CIS (x) or sourcee | Family member identified in other studies | Unique database identifier | |
|---|---|---|---|---|---|---|---|---|---|
| Mouse | Human | ||||||||
| II-2Rα | 7 kb upstream of gene | C, E | 1# | IV | 2A1 | 10p15.1 | RTCGD | NM_008367 | |
| II-2Rβ | 500 bp upstream of gene | C, E | 1 | III | 15E2 | 22q13.1 | RTCGD | NM_008368 | |
| Notch-1 | Exon 12, intron 14 | C, E, N | 2 | II | 2A3 | 9q34.3 | RTCGD (31) | Notch-2 | NM_008714 |
| Flt3 | 1.5-2 kb upstream of gene | E | 2 | III | 5G2 | 13q12.3 | RTCGD | NM_010229 | |
| Tie-1 | 6-15 kb downstream of gene | C, E | 2 | III, V | 4D1 | 1p34.2 | x | NM_011587 | |
| c-Mpl | 0.25-10 kb upstream of gene | C, E | 2 | III, V | 4D1 | 1p34 | x | NM_010823 | |
| Edg3 (G protein coupled) | 18 kb upstream of gene | C, E | 7/12§ | II, III, IV | 13B1 | 9q22.1 | x | Edg1 (RTCGD) | NM_010101 |
| Lck-1 | Intron 1 (upstream of ATG) | C, E | 1 | III | 4D2.3 | 1p34.3 | RTCGD | XM_110515 | |
| Pim-1 | 9 kb downstream of gene | C, E | 1 | VI | 17B1 | 6p21.2 | RTCGD (50) | Pim-2, Pim-3 | NM_008842 |
| Akt-2 | 11-14 kb upstream of gene | C, E | 4/13 | II, IV | 7A3 | 19q13.1 | RTCGD | Akt-1 | NM_007434 |
| PTPN5 | Exon 1 (upstream of ATG) | C, E, N | 1/24 | III | 7B3 | 11p15.1 | x | PTPN18, PTPNS1 (RTCGD) | NM_013643 |
| PTPN1 (PTP1B) | Intron 1 (downstream of ATG) | C, E, N | 9/13 | II, III, IV | 2H3 | 20q13.13 | RTCGD | PTPN18, PTPNS1 | NM_011201 |
| DUSP10 (MKP-5) | 1 kb upstream of gene | C, E | 1 | III | 1H4 | 1q41 | RTCGD | DUSP2 | NM_022019 |
| Inpp5b | 0.3 kb upstream of gene | C, E | 6/13 | I, III, V | 4D1 | 1p34.3 | x | NM_008385 | |
| Inpp4a | Intron 14 | C, E, N | 6/12 | III, IV, V | 1B | 2q11.2 | RTCGD | NM_030266 | |
| Dok-1 (p62) | 26-28 kb from gene | C, E, N | 10/10 | I, II, III | 6D1 | 2p13 | x | Dok1 (pending) (RTCGD) | NM_001381 |
| Phospholipase D3 (Pld3) | 25 kb upstream of gene | E | 5/13 | II, III, IV | 7A3 | 19q13.1 | RTCGD | Plaa, 2310004B05Rik | NM_011116 |
| Dapp-1 | 0.8 kb upstream of gene to intron 1 (downstream of ATG) | C, E | 11/13 | II, III, IV | 3H2 | 4q24 | RTCGD | NM_011932 | |
| Edaradd | 17 kb downstream of gene | C, E | 1 | III | 13A2 | 1q43 | RTCGD | NM_133643 | |
| Semaphorin 4b | 3 kb upstream of gene | C, E | 1 | V | 7D2 | 15q26.1 | RTCGD | Semaphorin 7a | XM_133534 |
| Insulin receptor substrate 2 | 2.5 kb downstream of gene | C, E | 2 | III, IV | 8A2 | 13q34 | RTCGD | XM_146235 | |
| NF1 (Evi-2) | Intron 35 | C, E | 2 | III | 11B5 | 17q11.2 | RTCGD (5) | NM_010161 | |
| PKI-gamma | 66 kb upstream of gene | C, E | 1 | IV | 2H3 | 20q13.12 | RTCGD | U97170 | |
| Tao-2 | 3-4 kb upstream of gene | C, E | 5/13 | II, III, IV | 7F4 | 16p13.11 | RTCGD | NM_004783 | |
| Shc3 | 41 kb downstream of gene | C, E | 7/12 | II, III, IV | 13B1 | 9q22.1 | x | NM_009167 | |
| Calcyphosine | Intron 4 (downstream of ATG) | C, E | 2/13 | II | 15A2 | 5p13.3 | x | NM_029341 | |
| TNF-related protein 6 | 2.5 kb downstream of gene | C, E | 1 | III | 15E2 | 22q13.1 | RTCGD | Many | AF329842 |
| Lymphotoxin beta (TNF-C) | 8.3 kb upstream of gene | C, E | 1 | III | 17B2 | 6p21.3 | RTCGD | Many | NM_008518 |
| Phosphodiesterase 1 | Intron 3, intron 4 (downstream of ATG) | C, E | 6/13 | I, II, III, IV | 10A3 | 6q22.31 | x | Phosphodiesterase 4b (RTCGD) | NM_006208 |
| Socs-2 | 0.75 kb upstream of gene to intron 1 (downstream of ATG) | C | 8/39 | II, III, IV, V | 10C3 | 12q22 | x | Socs-1, Socs-7, Asb-2 (RTCGD) | NM_003877 |
| ARF-related protein | 1.5 kb upstream of gene, intron 1 (upstream of ATG) | C, E | 8/13 | II, III, IV | 2H4 | 20q13.13 | x | ARF6, ARF2 (RTCGD) | AF217796 |
| RAP-1 | 18 kb upstream of gene | C, E | 1 | III | 15F2 | 12q12 | x | RAP1a (RTCGD) | AF103905 |
a
IL-2Rα, interleukin 2 receptor alpha; PKI-gamma, phosphokinase gamma; TNF, tumor necrosis factor; ARF, ADP-ribosylation factor.
b
Sequences from inverse PCR were analyzed with Celera (C), Ensembl (E), and National Center for Biotechnology Information (N) BLAST search programs, and the exact integration site was localized.
c
Number of tumors found positive for integration by inverse PCR only (out of 69 analyzed) # or by inverse PCR followed by directed PCR§ (positive out of total analyzed).
e
Source (reference) for other studies in which the Gr-1.4 virus integration site was previously found.