Table 1.
Antibodies used for immunocytochemical characterisation of cultured RBECs and RSCECs
| Antigen | Species | Manufacturer | Concentration |
|---|---|---|---|
| Caveolin 1 |
Rabbit |
Abcam ab2910 |
20 μg/ml |
| Clathrin heavy chain |
Rabbit |
Abcam ab21679 |
20 μg/ml |
| Claudin-5 |
Mouse |
Life Technologies 35-2500 |
10 μg/ml |
| Occludin |
Mouse |
Life Technologies 33-1500 |
10 μg/ml |
| P-gp |
Mouse |
Abcam ab3366 |
3.35 μg/ml |
| Smooth muscle actin |
Mouse |
R & D Systems MAB1420 |
4 μg/ml |
| Von Willebrand factor |
Rabbit |
Abcam ab6994 |
156 μg/ml |
| ZO-1 | Rabbit | Abcam ab59720 | 10 μg/ml |