FIG. 3.

Effects of BDCRB on intracellular GPCMV DNA. (A) Replicate cultures were infected with equal inocula of GPCMV and incubated in the presence of the indicated doses of BDCRB. Intracellular DNA was prepared at 4 days p.i., separated by FIGE, transferred to nylon membranes, and hybridized with the O probe to detect GPCMV replicative DNA forms. (B) Replicate cultures were infected in the presence or absence of 50 μM BDCRB. At 4 days p.i., the cells were subjected to hypotonic lysis and Dounce homogenization. The lysates were then either mock treated (−) or treated with staphylococcal nuclease (+) prior to DNA preparation and analysis as described for panel A. The locations of viral concatemeric and genomic 230-kb DNAs are indicated.