FIG. 5.

Effects of BDCRB on the right end of the GPCMV genome. Replicate cultures were infected for 4 days in the presence of the indicated doses of BDCRB (micromolar). Intracellular concatemeric and 230-kb DNAs were separated by FIGE and excised from the gel in agarose blocks. Virion DNA was prepared from culture supernatants by centrifugation. All DNAs were then digested with HindIII, separated by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with the O probe to detect terminal M and O fragments. Arrows indicate the HindIII M and O terminal fragments as well as the O-R junction fragment present in concatemeric DNA. A schematic below illustrates the right ends of type I and type II genomes and shows the HindIII sites (H) that define the HindIII O and M terminal fragments. The terminal repeat is indicated by a hatched box. A thick line indicates the sequences contained within the O probe.