Figure 2. A Mitochondrial Superoxide Signal Extends CLS.

(A) Western blot analysis of Sod2p (MnSOD) in P_TDH::SOD2 and wild-type (DBY2006). Samples were collected at an OD₆₀₀ of 1.0. MnSOD band intensity was normalized to actin, and the DBY2006 protein level was set to one. Values under the blot represent the mean ±SEM for three biological replicates.

(B) CLS in wild-type and P_TDH::SOD2 strains following exponential phase treatment with menadione (MD) or ethanol (nt).

(C) CLS in wild-type and sod2Δ strains as in (B).

(D) CLS in wild-type and rho⁰ as in (B).