Figure 3. Hormetic mtROS Longevity Signaling Requires the Tel1p and Rad53p DNA-damage Response Kinases.
(A) Schematic of DNA-damage signaling in Saccharomyces cerevisiae.
(B) CLS of sml1Δ and sml1Δ/rad53Δ strains treated with 50 μM menadione (MD) or vehicle (nt) during exponential growth.
(C) ROS levels in sml1Δ and sml1Δ/rad53Δ strains treated with menadione (+) or vehicle (−) during exponential growth as in Figure 1D. Samples were collected at day one of CLS.
(D) CLS of wild-type (DBY2006) and tel1Δ strains as in (B). See also Figure S2.