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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Cell Metab. 2013 Jun 4;17(6):954–964. doi: 10.1016/j.cmet.2013.04.003

Figure 5. mtROS Regulate Rph1p to Modify Subtelomeric Heterochromatin.

Figure 5

(A) Schematic of Rad53p-dependent regulation of Rph1p chromatin binding and histone demethylase activity.

(B) Western blot analysis of Rph1-HA phosphorylation in wild-type, tel1Δ and sml1Δ/rad53Δ strains treated with 50 μM menadione or 0.01% MMS. Samples for western blots were collected after 30 minutes of treatment during exponential growth.

(C) ChIP of Rph1-HA at a control region (ACT1) and two subtelomeric genes (YPS6 and YEL077C) in a wild-type or sml1Δ/rad53Δ background at day one of CLS. Data points represent the mean of two independent ChIP experiments, error bars represent the range. Values were normalized to the wild-type untreated ACT1 sample, which was set to one.

(D) ChIP using antibodies against trimethyl H3K36 or total H3 in wild-type or rph1Δ as in (C). See also Figure S4.