Skip to main content
. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Cell Metab. 2013 Jun 4;17(6):954–964. doi: 10.1016/j.cmet.2013.04.003

Figure 7. mtROS Signaling via Rph1p Promotes Sir3p Binding at Subtelomeres to Repress Gene Expression and Extend CLS.

Figure 7

(A) ChIP of Sir3p in wild type (DBY2006) and rph1Δ at a control region (ACT1) and two subtelomeric genes (YPS6 and YEL077C). Cells were analyzed at day one of CLS following treatment with 50 μM menadione or vehicle during exponential growth.

(B) Quantitative RT-PCR of subtelomeric transcripts relative to ACT1 at day one of CLS in wild-type or sir3Δ yeast following 50 μM menadione or vehicle during exponential growth.

(C) CLS of wild-type and sir3Δ treated with 50 μM menadione (MD) or vehicle (nt) during logarithmic growth.

(D) Model for mtROS signaling via DNA damage response kinases and Rph1p to regulate subtelomeric heterochromatin formation and CLS.