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Analysis of meiosis proteins by western blot analysis. Western blot analysis was used to measure the expression of SPO11 (Abcam, ab81695), REC8 (Sigma, HPA031729), SGO2 (Sigma, HPA035163), and HORMAD1 (Abcam, ab57883) in melanoma lines compared to nontransformed melanocytes, fibroblasts, and keratinocytes. The cytoplasmic β-actin for each experiment is shown below its respective blot as a loading control.
