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T helper type 17 (Th17) cells from psoriatic lesions can produce the Th1 cytokine interferon (IFN)-γ and are all of the activated CD45RO phenotype. Representative flow cytometric plots of paired peripheral blood mononuclear cells (PBMC) and lesional CD4+ T cells showing staining for interleukin (IL)-17A and IFN-γ (a) after 12-h culture with Brefeldin A, or expression of the activation or memory marker CD45RO by IL-23R+ gated Th17 phenotype cells (b).
