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. 2013 Jun 6;173(1):47–57. doi: 10.1111/cei.12089

Figure 6.

Figure 6

Effect of let-7i mimic transfection on Toll-like receptor (TLR)-4 mRNA and protein expression. Let-7i mimic was transfected into Jurkat cells via electroporation. (a) After let-7i mimic transfection for 24 h, the expression level of let-7i was increased dramatically in the Jurkat cells compared with the scrambled oligonucleotides transfection group. (b) The TLR-4 mRNA expression levels did not change after let-7i transfection. (c) Jurkat cells were cultured with 250 ng/ml ionomycin and 10 ng/ml phorbol myristate acetate (PMA) and normal T cells were cultured with anti-CD3+anti-CD28 antibodies for 24 h after let-7i mimic transfection. The protein expression of TLR-4 was inhibited significantly in Jurkat and normal T cells after let-7i mimic transfection. (d) A representative case showing increased TLR-4 protein expression after let-7i mimic transfection into Jurkat (left) and normal T cells (right).