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. 2013 Jun 6;173(1):47–57. doi: 10.1111/cei.12089

Figure 8.

Figure 8

Functional analysis of let-7i in anti-CD3+anti-CD28-activated normal and ankylosing spondylitis (AS) T cells via transfection with let-7i mimic and inhibitor. (a) Lipopolysaccharide (LPS) (100 ng/ml) potently suppress interferon (IFN)-γ production in anti-CD3+anti-CD28 activated T cells from healthy volunteers but not AS patients. *The IFN-γ concentration was lower in anti-CD3+anti-CD28+ LPS-stimulated normal T cells compared with AS T cells. (P < 0·05) (b) The transfection of let-7i mimic increased IFN-γ production in anti-CD3+anti-CD28+ LPS-activated T cells from healthy volunteers, but not AS patients. The transfection of let-7i inhibitor suppressed IFN-γ production by anti-CD3+anti-CD28+ LPS-stimulated AS T cells, but not normal T cells. *In the scrambled oligonucleotides transfected control groups, the IFN-γ concentration was higher in anti-CD3+anti-CD28+ LPS-stimulated AS T cells compared with normal T cells (P < 0·05).