Figure 1.

Bmal1 suppresses A549 lung cancer cell invasion. (A) Human A549 lung cancer cells were transfected with the empty (control) or Bmal1-expressing pCMV-SPORT6 vectors. Where indicated, the cells were alternatively treated with Bmal1-targeting siRNAs. After a 46-h incubation, the cellular levels of Bmal1 were analyzed by western blotting, using β-actin as a loading control. (B) The invasiveness of control and Bmal1-overexpressing cells was compared using Matrigel-coated membranes. (C) The invasiveness of cells transfected with either control or Bmal1-targeting siRNAs was compared. (D) A549 cells were treated with control siRNAs or those against Bmal1, Per3, or RORα, and cellular invasiveness was compared. The graphs show the means and SD from three independent experiments. ^*P>0.05 vs. control siRNA.