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. 2004 Feb;78(4):1675–1684. doi: 10.1128/JVI.78.4.1675-1684.2004

FIG. 1.

FIG. 1.

Expression of ISG56 is only observed in the absence of ICP0 expression. (A) Western blot analysis of whole-cell extracts (40 μg) of HEL fibroblasts harvested 12 h postinfection with the indicated viruses. The ISG56 protein is only visible following infection with UV-inactivated wild-type virus (strain KOS, WT-UV) or an ICP0 null mutant (n212). (B) RT-PCR analysis of total RNA harvested from HEL fibroblasts at various times postinfection with the indicated viruses. In the absence of ICP0, transient ISG56 mRNA accumulation is seen, whereas in the absence of HSV-1 replication (UV-inactivated KOS or genetically inactivated KM110), sustained ISG56 mRNA accumulation is observed. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.