Table 2.

Clinical data (cyanosis assessment and modified-SHIRPA test) on SAG-TD– and SAG-HD–treated mice. All the pups treated with SAG-HD were monitored every day for cyanosis. The color of the skin is indicated in the table for each day of injection. The SAG-TD– and SAG-HD–treated animals were tested at 1 and 6 months of age, respectively, for behavioral, morphological, or performance changes using the modified-SHIRPA protocol. N, normal; NP, not present; P, present.

Age		1 month			6 months
Treatment (P0 to P8)		Vehicle	SAG-TD	SAG-TD + prednisolone	Vehicle	SAG-HD
n	Males	1	2	1	1	1
	Females	2	1	2	—	—
Category	Tests
Coat	Coat color; hair length; hair morphology; skin color; piloerection	N	N	N	N	N
Morphology	Tail, pinna, head, limb, whisker, and teeth morphology; palpebral closure	N	N	N	N	Abnormal head morphology
Posture	Body position; body, limb, and abdominal tone; visual placing; tail and pelvic elevation; trunk curl	N	N	N	N	N
Activity	Spontaneous and locomotor activity; wire maneuver; negative geotaxis; gait	N	N	N	N	Poor negative geotaxis
Behavior	Fear; irritability; aggression; vocalization; bizarre behavior; convulsions; provoked biting; tremor	NP	NP	NP	NP	NP
Reflexes	Limb grasping; grip strength; pinna and corneal reflex; toe pinch; touch escape; transfer arousal; contact righting	P	P	P	P	Semi-effective grip
Body releases	Defecation; urination; lacrimation; salivation	N	N	N	N	N
Heart rate		N	N	N	N	N
Respiration rate		N	N	N	N	N
Body weight (g)	Males	29.3	29.1 ± 0.6	28.9	48.5	28
	Females	26.0 ± 1.113	25	25.1 ± 0.4	—	—
Body length (mm)	Males	105	100 ± 0	105	120	90
	Females	102.5 ± 3.5	95	100 ± 0	—	—
Tail length (mm)	Males	95	95 ± 7.1	85	75	80
	Females	90.0 ± 7.1	90	95 ± 0	—	—

Age analysis	P1		P2		P3		P4		P5		P6		P7
Treatment (P0 to P8)	Vehicle	SAG-HD	Vehicle	SAG-HD	Vehicle	SAG-HD	Vehicle	SAG-HD	Vehicle	SAG-HD	Vehicle	SAG-HD	Vehicle	SAG-HD
n	6	8	5	8	4	7	4	7	4	7	3	7	3	7
Skin color	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink	Pink